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The double helix model was proposed by


A) Watson and Crick.
B) Griffith.
C) Avery.
D) Franklin.
E) Beadle and Tatum.

F) All of the above
G) D) and E)

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If an


A) Repair would take place through replication and recombination during sister chromatid paring.
B) Inaccurate replication of the genome would eventually result in cell death.
C) uvr B or C would handle the repair of the thymine dimer.
D) It would have no lasting effects.
E) coli strain were phr (no photoreactivation) ,uvrA (no excision repair) ,and recA (no SOS repair) ,what do you expect would be the result of thymine dimer formation?

F) A) and D)
G) A) and E)

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Since both the strands of a DNA molecule are complimentary to each other,either one of the strands can be used as a _______ to reconstruct the other.


A) copy
B) basis
C) compass
D) template

E) A) and D)
F) All of the above

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In 1952,Hershey and Chase confirmed Avery's (1944) results that


A) proteins were the repositories for hereditary information.
B) DNA was the repository for hereditary information.
C) RNA was the repository for hereditary information.
D) proteins and DNA were the repositories for hereditary information.
E) transformation in bacterial cells induced changes in the host's somatic cells.

F) None of the above
G) A) and B)

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Fourteen percent of the DNA nucleotides from a certain organism contain T.What amounts of the other bases would you expect to be present in this particular DNA?


A) 14 % A,0% C,36 % G
B) 14 % A,36 % C,14 % G
C) 14 % A,36 % C,36 % G
D) 14 % T,38 % C,36 % G
E) 14 % T,36 % C,36 % U

F) None of the above
G) A) and D)

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Replication always proceeds by adding new bases to the


A) 1' end.
B) 2' end.
C) 3' end.
D) 4' enD.
E) 5' end.

F) A) and D)
G) A) and C)

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If the Ξ²\beta subunit of DNA pol III had a mutation that affected its sliding clamp configuration,which of the following would be most affected?


A) DNA unwinding
B) supercoiling
C) proper formation of the replication fork
D) processivity

E) None of the above
F) A) and B)

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Deoxyribose has a "free arm," a carbon that is not a member of the pentose ring.To which direction does this "free arm" face?


A) in the same direction as which the coding strand is read
B) in the opposite direction from which the coding strand is read
C) toward the nitrogenous bases
D) toward the O in the five carbon ring

E) B) and C)
F) A) and D)

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___ is(are) the genetic material for all cellular organisms and some viruses.


A) RNA
B) DNA
C) Mitochondria
D) Chromosomes

E) B) and D)
F) B) and C)

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The processivity of a polymerase is a measure of how fast it can copy a DNA template.A polymerase with a high processivity has a high speed,while a polymerase with a low processivity has a slow speed.Given this information,how does processivity relate to the sensitivity and error rate of a polymerase?


A) high processivity = high sensitivity and high error rate
B) high processivity = high sensitivity and low error rate
C) high processivity = low sensitivity and high error rate
D) high processivity = low sensitivity and low error rate

E) B) and C)
F) A) and D)

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Which of the following statements about the Hershey-Chase experiment is false?


A) DNA was labeled with radioactive phosphorus.
B) Protein was labeled with radioactive sulfur.
C) The virus-infected bacteria showed radioactive phosphorus.
D) The virus-infected bacteria contained radioactive sulfur.

E) B) and C)
F) None of the above

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As the two strands of DNA are unraveled,an enzyme relieves the strain on the two strands.The enzyme is


A) DNA polymerase.
B) DNA ligase.
C) DNA gyrase.
D) DNA endonuclease.
E) DNA exonucleasE.

F) C) and E)
G) All of the above

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When DNA is replicated during S of the cell cycle,the sequence is maintained but the double strand is not.Each strand of the original double strand accompanies its new complement strand.This process is known as the


A) disruptive DNA replication.
B) conservative DNA replication.
C) semiconservative DNA replication.
D) dispersive DNA replication.
E) stabilizing DNA replication.

F) A) and C)
G) B) and C)

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What would be the effect on DNA replication if the helicase was unable to hydrolyze ATP?


A) Helicases use the energy of ATP hydrolysis to unwind the strands of DNA during replication.Although,some DNA polymerases can unwind DNA as they synthesize new DNA,helicases make the process is much more efficient.If helicases cannot hydrolyze ATP,they cannot properly unwind the DNA,and replication would be slowed.
B) Helicases use the energy of ATP hydrolysis to wind the strands of DNA during replication.Although,some DNA polymerases can wind DNA as they synthesize new DNA,helicases make the process is much more efficient.If helicases cannot hydrolyze ATP,they cannot properly wind the DNA,and replication would be slowed.
C) Helicases unwind the strands of DNA during replication.If helicases cannot hydrolyze ATP,they cannot unwind the DNA,and replication would be stopped.

D) A) and C)
E) B) and C)

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Hershey-Chase concluded from their experiment with T2 bacteriophage that


A) proteins are the genetic material.
B) polysaccharide coat contains the genetic material.
C) DNA is the genetic material.
D) viruses are needed for DNA to function.
E) DNA contains sulfur.

F) A) and C)
G) None of the above

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The method of DNA replication,where each original strand is used as a template to build a new strand,is called the


A) conservative method.
B) semiconservative method.
C) disruptive method.
D) continuous methoD.
E) replication and amplification method.

F) C) and D)
G) B) and D)

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Which of the following enzymes removes the primers used for DNA replication?


A) DNA pol I
B) DNA pol II
C) DNA pol III
D) DNA pol I,DNA pol II,and DNA pol III can all remove primers

E) All of the above
F) A) and D)

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The DNA double helix is similar to a twisted ladder.What makes up the sides holding the rungs together?


A) phosphate groups only
B) alternating units of phosphate groups and sugars
C) sugars only
D) purines and pyrimidines

E) B) and D)
F) C) and D)

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In Griffith's transformation experiments,


A) mice infected with live R form caused pneumonia and death in the mice.
B) the R form transformed the S form into a virulent variety.
C) mice infected with heat-killed S form and live R form caused pneumonia and death in the mice.
D) the S form lacked an enzyme involved in the manufacture of the polysaccharide coat,leading to the abnormally smooth colonies.

E) All of the above
F) A) and B)

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Why does DNA pol I carry the number one when it is not the polymerase responsible for the bulk of DNA synthesis?


A) DNA pol I was the first polymerase isolated from
B) DNA pol I is the first polymerase to be activated during DNA replication.
C) DNA pol I is the only polymerase that has both 3' to 5' and 5' to 3' exonuclease activity.
D) DNA pol I is the smallest polymerase.
E) coli.

F) B) and C)
G) B) and E)

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