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Geneticists can create sequences of DNA from RNA using enzymes called


A) palindromes.
B) reverse transcriptases.
C) restriction endonucleases.
D) ligases.
E) DNA polymerases.

F) A) and B)
G) B) and E)

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An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

A) True
B) False

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Identification of unique DNA fingerprints relies on the presence of single nucleotide polymorphisms among samples.

A) True
B) False

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Transgenic animals


A) can be engineered to become factories for manufacturing proteins.
B) are often obtained from germline engineering.
C) will pass the genes on to their offspring.
D) commonly include mice.
E) All of the choices are correct.

F) C) and E)
G) B) and E)

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When patient tissues are transfected with viruses carrying a needed,normal human gene,the technique is called


A) cloning.
B) gene therapy.
C) antisense therapy.
D) DNA fingerprinting.

E) A) and B)
F) None of the above

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This process is often used in forensic science in order to distinguish one sequence of DNA from another by comparing the sequence of the strands at specific loci:


A) cloning
B) gene therapy
C) antisense therapeutic
D) DNA fingerprinting

E) None of the above
F) A) and B)

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Vectors often contain a gene conferring drug resistance to their cloning host,in order to detect cells harboring the plasmid.

A) True
B) False

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DNA strands can begin to separate at the temperature of:


A) 37oC.
B) 42oC.
C) 60oC.
D) 90oC.
E) 100oC.

F) A) and B)
G) A) and C)

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Your infant has been diagnosed with severe combined immunodeficiency disease (SCID) .Your doctor suggests which treatment as a possible cure for the disease


A) gene therapy
B) genome mapping
C) reverse transcription
D) microarrays

E) C) and D)
F) B) and D)

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DNA fragments can be separated in gel electrophoresis because


A) nitrogenous bases have a net positive charge.
B) nitrogenous bases have a net negative charge.
C) phosphate groups have a net positive charge.
D) phosphate groups have a net negative charge.

E) A) and D)
F) A) and C)

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Labeled,known,short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are known as


A) genetic engineering.
B) biotechnology.
C) recombinant DNA.
D) gel electrophoresis.
E) gene probes.

F) A) and C)
G) A) and E)

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Of the following choices,which could be used in the treatment of a patient in order to determine the patient's cancer subtype?


A) transformation
B) PCR
C) microarray analysis
D) Oryza sativa
E) Western Blot analysis

F) C) and E)
G) A) and E)

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