Question 1

Which PCR step causes the denaturation of double-stranded DNA?

Accepted Answer

A)  add DNA polymerase and nucleotides at 72 ∘\circ∘ C
B)  cool DNA to between 50 ∘\circ∘ C and 65 ∘\circ∘ C
C)  add primers
D)  heat target DNA to 94 ∘\circ∘ C
E)  repeat the cycle of heating and cooling F) A) and B)
G) A) and C)

Question 2

Which of the following is less subject to degradation than is chromosomal DNA and is used as an evolutionary time clock?

Accepted Answer

A)  mitochondrial DNA
B)  chloroplast DNA
C)  rRNA
D)  mRNA
E)  tRNAF) D) and E)
G) A) and B)

Question 3

Compare and contrast recombinant DNA and gene therapy.

Accepted Answer

Recombinant DNA and gene therapy are bot...

Question 4

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called

Accepted Answer

A)  cloning.
B)  gene therapy.
C)  antisense therapeutic.
D)  DNA fingerprinting.
E)  None of the choices are correct.F) B) and E)
G) B) and C)

Question 5

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Accepted Answer

A) True 
 B)False

Question 6

An example of gene therapy is the insertion of the gene for human growth hormone into
E.coli cells.

Accepted Answer

A) True 
 B)False

Question 7

The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA technology.
D)  gel electrophoresis.
E)  gene probes.F) A) and E)
G) B) and D)

Question 8

One of the first uses of DNA fingerprinting was in _________ medicine.

Accepted Answer

The answer of One of the first uses of DNA...

Question 9

Sequences of DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are

Accepted Answer

A)  palindromes.
B)  reverse transcriptases.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) A) and C)
G) C) and D)

Question 10

Analysis of DNA fragments in gel electrophoresis is based on:

Accepted Answer

A)  larger fragments move slowly and remain closer to the wells.
B)  DNA has an overall negative charge and moves to the positive pole.
C)  DNA fragments are stained to see them.
D)  an electric current through the gel causes DNA fragments to migrate.
E)  All of the choices are correct.F) B) and E)
G) None of the above

Question 11

Genomic _____ are collections of isolated genes maintained in a cloning host.

Accepted Answer

The answer of Genomic _____ are collections of isolated genes...

Question 12

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

Accepted Answer

The answer of The two common vectors used to transfer...

Question 13

Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects:

Accepted Answer

A)  Escherichia coli
B)  Saccharomyces cerevisiae
C)  Thermus aquaticus
D)  Pseudomonas syringae
E)  Pseudomonas fluorescensF) A) and D)
G) B) and E)

Question 14

Amplification of DNA is accomplished by

Accepted Answer

A)  polymerase chain reaction.
B)  DNA sequencing.
C)  gene probes.
D)  Southern blot.
E)  All of the choices are correct.F) A) and E)
G) All of the above

Question 15

The _____ blot method analyzes DNA, while the _____ method analyzes RNA.

Accepted Answer

The answer of The _____ blot method analyzes DNA, while...

Question 16

Which of the following methods determines which genes are actively transcribed in a cell under a variety of conditions?

Accepted Answer

A)  DNA fingerprinting
B)  microarray analysis
C)  gene mapping
D)  Western blot
E)  antisense therapyF) A) and B)
G) All of the above

Question 17

All of the following are characteristics that make plasmids good cloning vectors, except

Accepted Answer

A)  they are small.
B)  they are well characterized.
C)  they can be transferred into appropriate host cells.
D)  they can carry genetic markers, such as antibiotic resistance genes.
E)  they can accept a relatively large amount of foreign DNA.F) A) and B)
G) A) and E)

Question 18

Which of the following is a serious concern when performing PCR?

Accepted Answer

A)  high temperature needed may denature the DNA
B)  restriction enzymes are difficult to obtain in adequate quantities
C)  introduction and amplification of contaminating DNA
D)  exposure to radiation by the lab personnel
E)  it is a very time consuming processF) C) and D)
G) A) and B)

Question 19

When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA?

Accepted Answer

A)  PCR
B)  Cloning
C)  The Sanger Method
D)  Southern blot hybridization
E)  Microarray analysisF) A) and B)
G) B) and E)

Question 20

In order to ensure compatibility between the target DNA and the plasmid DNA,

Accepted Answer

A)  target DNA is removed from cells and isolated.
B)  cloning host is treated with calcium chloride and receives plasmid.
C)  target DNA and plasmid are treated with the same restriction endonuclease.
D)  desired protein is produced by cloning host.
E)  gene is amplified by multiplication of cloning host.F) C) and D)
G) D) and E)

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Genomic _____ are collections of isolated genes maintained in a cloning host.

Correct AnswerverifiedShow Answer

Sequences of DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are

Correct AnswerverifiedShow Answer

Which of the following methods determines which genes are actively transcribed in a cell under a variety of conditions?

Correct AnswerverifiedShow Answer

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called

Correct AnswerverifiedShow Answer

In order to ensure compatibility between the target DNA and the plasmid DNA,

Correct AnswerverifiedShow Answer

Which PCR step causes the denaturation of double-stranded DNA?

Correct AnswerverifiedShow Answer

Amplification of DNA is accomplished by

Correct AnswerverifiedShow Answer

Recombinant strains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects:

Correct AnswerverifiedShow Answer

One of the first uses of DNA fingerprinting was in _________ medicine.

Correct AnswerverifiedShow Answer

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

Correct AnswerverifiedShow Answer

The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called

Correct AnswerverifiedShow Answer

Which of the following is less subject to degradation than is chromosomal DNA and is used as an evolutionary time clock?

Correct AnswerverifiedShow Answer

When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA?

Correct AnswerverifiedShow Answer

Analysis of DNA fragments in gel electrophoresis is based on:

Correct AnswerverifiedShow Answer

The _____ blot method analyzes DNA, while the _____ method analyzes RNA.

Correct AnswerverifiedShow Answer

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Correct AnswerverifiedShow Answer

Which of the following is a serious concern when performing PCR?

Correct AnswerverifiedShow Answer

All of the following are characteristics that make plasmids good cloning vectors, except

Correct AnswerverifiedShow Answer

Compare and contrast recombinant DNA and gene therapy.

Correct AnswerAnswered by ExamLex AIRecombinant DNA and gene therapy are bot...View AnswerShow Answer

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

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The _ blot method analyzes DNA, while the _ method analyzes RNA.

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Answered by ExamLex AI
Recombinant DNA and gene therapy are bot...
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The two common vectors used to transfer a piece of DNA into a cloning host are _ and _.

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