Question 1

When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

Accepted Answer

A) True 
 B)False

Question 2

The tuberculin test is an example of an in vivo serological test.

Accepted Answer

A) True 
 B)False

Question 3

Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?

Accepted Answer

A)  Ouchterlony double diffusion
B)  Western blot
C)  immunelectrophoresis
D)  radioimmunoassay (RI
A)  
E)  the Quellung testF) A) and B)
G) A) and C)

Question 4

In a __________, antigens or antibodies are labeled with radioactive isotopes and traced.

Accepted Answer

The answer of In a __________, antigens or antibodies are...

Question 5

A simple method to detect _____ cells involves mixing them with untreated sheep red blood cells that will bind and form rosettes.

Accepted Answer

A) True 
 B)False

Question 6

Affixing antigen to an inert particle such as a latex bead is sometimes used in which type of test?

Accepted Answer

A)  cross-reactions
B)  agglutination
C)  precipitation
D)  specificity
E)  sensitivityF) A) and E)
G) A) and B)

Question 7

Precipitation tests involve all of the following, except:

Accepted Answer

A)  they rely on formation of visible clumps for detection.
B)  they include the VDRL test for syphilis.
C)  they are often performed in agar gels.
D)  they can be done in a test tube by carefully adding antiserum over antigen solution.
E)  a cloudy or opaque zone developing where antigen and antibody reactF) B) and E)
G) A) and B)

Question 8

Which of the following is incorrect regarding the complement fixation test?

Accepted Answer

A)  First antigen and antibody are allowed to react.
B)  Purified complement proteins are added to the antigen-antibody tube.
C)  Sheep red blood cells are added to the antigen-antibody-complement mixture.
D)  Hemolysis of the sheep red blood cells occurs in a positive test result.
E)  None of the choices are correct.F) B) and E)
G) A) and B)

Question 9

Sputum is obtained by:

Accepted Answer

A)  swabbing the mouth to sample saliva.
B)  a clean catch urine sample.
C)  the patient coughing up mucus from the lower respiratory system.
D)  a spinal tap above the chest.
E)  a throat swab.F) B) and E)
G) B) and D)

Question 10

When serum proteins are separated by electrophoresis and then antibodies specific for the serum proteins are placed in a parallel trough in order to form reaction arcs for each protein, the test is called:

Accepted Answer

A)  Ouchterlony double diffusion.
B)  Western blot.
C)  immunelectrophoresis.
D)  radioimmunoassay (RI
A)  .
E)  the Quellung test.F) B) and E)
G) A) and D)

Question 11

When antigen-antibody reactions are used to read a _____, they determine the quantity of antibodies in the serum.

Accepted Answer

The answer of When antigen-antibody reactions are used to read...

Question 12

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Accepted Answer

A) True 
 B)False

Question 13

Serotyping of the pneumococcus based on capsular polysaccharide is performed in:

Accepted Answer

A)  Ouchterlony double diffusion.
B)  Western blot.
C)  immunelectrophoresis.
D)  radioimmunoassay (RI
A)  .
E)  the Quellung test.F) A) and B)
G) A) and E)

Question 14

In _________ tests, antibody cross-links whole-cell antigens, forming complexes that settle out and form visible clumps in the test chamber.

Accepted Answer

The answer of In _________ tests, antibody cross-links whole-cell antigens,...

Question 15

When collecting __________, a "clean catch" method is often used.

Accepted Answer

The answer of When collecting __________, a "clean catch" method...

Question 16

Which of the following is involved in the in vitro diagnostic testing of serum?

Accepted Answer

A)  mycology
B)  hematology
C)  serology
D)  histology
E)  virologyF) D) and E)
G) A) and B)

Question 17

The physician orders an antistreptolysin O (ASO)  titer test to check for rheumatic fever.A patient serum sample is exposed to known suspensions of streptolysin and then allowed to incubate with RBCs? If the patient does have rheumatic fever, what will happen?

Accepted Answer

A)  Not enough information is provided to speculate.
B)  The red blood cells will lyse.
C)  The red blood cells will agglutinate only.
D)  The red blood cells will not lyse.
E)  The red blood cells will fluoresce.F) A) and B)
G) B) and E)

Question 18

All of the following are routine biochemical tests to aid in identifying a species, except:

Accepted Answer

A)  motility.
B)  carbohydrate fermentation.
C)  catalase test.
D)  oxidase test.
E)  coagulase test.F) A) and B)
G) D) and E)

Question 19

Soluble antigens are detected in which type of test?

Accepted Answer

A)  cross-reactions
B)  agglutination
C)  precipitation
D)  specificity
E)  sensitivityF) A) and D)
G) All of the above

Question 20

Maggie was told she has a positive titer to measles.Which of the following could explain this?

Accepted Answer

A)  She was vaccinated against measles.
B)  She had measles sometime in her life.
C)  She has measles now.
D)  The test was a false positive.
E)  All of the choices are correct.F) A) and B)
G) A) and E)

Exam 17: Procedures for Identifying Pathogens and Diagnosing Infections

All of the following are routine biochemical tests to aid in identifying a species, except:

Correct AnswerverifiedShow Answer

Maggie was told she has a positive titer to measles.Which of the following could explain this?

Correct AnswerverifiedShow Answer

Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?

Correct AnswerverifiedShow Answer

In a __________, antigens or antibodies are labeled with radioactive isotopes and traced.

Correct AnswerverifiedShow Answer

Which of the following is incorrect regarding the complement fixation test?

Correct AnswerverifiedShow Answer

A simple method to detect _____ cells involves mixing them with untreated sheep red blood cells that will bind and form rosettes.

Correct AnswerverifiedShow Answer

Sputum is obtained by:

Correct AnswerverifiedShow Answer

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Correct AnswerverifiedShow Answer

When antigen-antibody reactions are used to read a _____, they determine the quantity of antibodies in the serum.

Correct AnswerverifiedShow Answer

Precipitation tests involve all of the following, except:

Correct AnswerverifiedShow Answer

The physician orders an antistreptolysin O (ASO) titer test to check for rheumatic fever.A patient serum sample is exposed to known suspensions of streptolysin and then allowed to incubate with RBCs? If the patient does have rheumatic fever, what will happen?

Correct AnswerverifiedShow Answer

The tuberculin test is an example of an in vivo serological test.

Correct AnswerverifiedShow Answer

Serotyping of the pneumococcus based on capsular polysaccharide is performed in:

Correct AnswerverifiedShow Answer

When serum proteins are separated by electrophoresis and then antibodies specific for the serum proteins are placed in a parallel trough in order to form reaction arcs for each protein, the test is called:

Correct AnswerverifiedShow Answer

When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

Correct AnswerverifiedShow Answer

Which of the following is involved in the in vitro diagnostic testing of serum?

Correct AnswerverifiedShow Answer

Soluble antigens are detected in which type of test?

Correct AnswerverifiedShow Answer

In _________ tests, antibody cross-links whole-cell antigens, forming complexes that settle out and form visible clumps in the test chamber.

Correct AnswerverifiedShow Answer

Affixing antigen to an inert particle such as a latex bead is sometimes used in which type of test?

Correct AnswerverifiedShow Answer

When collecting __________, a "clean catch" method is often used.

Correct AnswerverifiedShow Answer

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