Question 1

Gene probes can be labeled for detection with

Accepted Answer

A)  enzymes.
B)  fluorescent dyes.
C)  radioisotopes.
D)  All of the choices are correct.
E)  None of the choices are correct.F) None of the above
G) All of the above

Question 2

Sequencing a gene automatically identifies which gene it is.

Accepted Answer

A) True 
 B)False

Question 3

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called

Accepted Answer

A)  cloning.
B)  gene therapy.
C)  antisense therapeutic.
D)  DNA fingerprinting.
E)  None of the choices are correct.F) A) and C)
G) None of the above

Question 4

When DNA is heated to just below boiling (90oC - 95oC) ,

Accepted Answer

A)  the sugar phosphate backbone of the DNA strands breaks.
B)  the sugar, phosphate and base come apart in each nucleotide.
C)  the helix unwinds but hydrogen bonds between the bases remain intact.
D)  the two DNA strands separate completely.
E)  Nothing happens until the boiling point is reached (100o
C)  .F) B) and E)
G) A) and E)

Question 5

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Accepted Answer

A) True 
 B)False

Question 6

The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA technology.
D)  gel electrophoresis.
E)  gene probes.F) A) and B)
G) A) and C)

Question 7

Some genes in bacteria are repressed by antisense RNA that binds to them.

Accepted Answer

A) True 
 B)False

Question 8

Sequences of DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are

Accepted Answer

A)  palindromes.
B)  reverse transcriptase.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) A) and D)
G) D) and E)

Question 9

An example of gene therapy is the insertion of the gene for human growth hormone into
E.coli cells.

Accepted Answer

A) True 
 B)False  False

Question 10

Transgenic animals are _____ modified organisms.

Accepted Answer

The answer of Transgenic animals are _____ modified organisms....

Question 11

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

Accepted Answer

The answer of The two common vectors used to transfer...

Question 12

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA.
D)  gel electrophoresis.
E)  gene probes.F) C) and E)
G) A) and B)

Question 13

The primers in PCR are

Accepted Answer

A)  synthetic DNA oligonucleotides.
B)  bacterial enzymes.
C)  short RNA strands.
D)  DNA polymerases.
E)  reverse transcriptases.F) A) and C)
G) All of the above A

Question 14

A technique that separates a readable pattern of DNA fragments is

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA.
D)  gel electrophoresis.
E)  gene probes.F) B) and C)
G) A) and B) D

Question 15

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plasmids or chromosomes.

Accepted Answer

The answer of In recombinant DNA technique, an enzyme called...

Question 16

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

Accepted Answer

A)  RNA promoters
B)  fluorescent nucleotides
C)  primers
D)  thermophilic polymerases
E)  endonucleasesF) A) and C)
G) A) and B)

Question 17

The Western Blot technique detects

Accepted Answer

A)  DNA.
B)  RNA.
C)  proteins.
D)  recombinant DNA.
E)  specific genetic marker sequence on genes.F) All of the above
G) C) and D)

Question 18

Which of the following is less subject to degradation than is chromosomal DNA and is used as an evolutionary time clock?

Accepted Answer

A)  mitochondrial DNA
B)  chloroplast DNA
C)  rRNA
D)  mRNAE) A) and B)
F) A) and C)

Question 19

The size of DNA is often given in the number of _____ that it contains.

Accepted Answer

The answer of The size of DNA is often given...

Question 20

Transformation and transfection are methods used to introduce DNA into host cells.

Accepted Answer

A) True 
 B)False

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Gene probes can be labeled for detection with

Correct AnswerverifiedShow Answer

Sequencing a gene automatically identifies which gene it is.

Correct AnswerverifiedShow Answer

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called

Correct AnswerverifiedShow Answer

When DNA is heated to just below boiling (90oC - 95oC) ,

Correct AnswerverifiedShow Answer

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Correct AnswerverifiedShow Answer

The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called

Correct AnswerverifiedShow Answer

Some genes in bacteria are repressed by antisense RNA that binds to them.

Correct AnswerverifiedShow Answer

Sequences of DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are

Correct AnswerverifiedShow Answer

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

Correct AnswerverifiedFalse

Transgenic animals are _____ modified organisms.

Correct AnswerverifiedShow Answer

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

Correct AnswerverifiedShow Answer

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are

Correct AnswerverifiedShow Answer

The primers in PCR are

Correct AnswerverifiedA

A technique that separates a readable pattern of DNA fragments is

Correct AnswerverifiedD

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plasmids or chromosomes.

Correct AnswerverifiedShow Answer

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

Correct AnswerverifiedShow Answer

The Western Blot technique detects

Correct AnswerverifiedShow Answer

Which of the following is less subject to degradation than is chromosomal DNA and is used as an evolutionary time clock?

Correct AnswerverifiedShow Answer

The size of DNA is often given in the number of _____ that it contains.

Correct AnswerverifiedShow Answer

Transformation and transfection are methods used to introduce DNA into host cells.

Correct AnswerverifiedShow Answer

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When DNA is heated to just below boiling (90^oC - 95^oC) ,

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False

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The two common vectors used to transfer a piece of DNA into a cloning host are _ and _.

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A

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D

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